To further explore the underlying mechanism of the selectivity, we explored the intermediates in this indole biodegradation pathway using liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS), which indicated that the indole biodegradation pathway in Providencia rettgeri is the catechol pathway. The heterologous expression of the indole degradation gene cluster in Escherichia coli BL21 (DE3) and in vitro enzymatic reaction demonstrated that the indole biodegradation gene cluster may contribute to selectively biosynthesizing Tyrian purple. An indole degradation gene cluster for indole metabolism was identified from this GS-2 strain. This GS-2 strain was then identified as Providencia rettgeri based on bacterial genome sequencing analysis. In this study, we found Tyrian purple can be selectively produced by a bacterial strain GS-2 when fed with 6-bromotryptophan in the presence of tryptophan. Tyrian purple, mainly composed of 6, 6′-dibromoindigo, is a precious dye extracted from sea snails. 4Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.3CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China. 2Shenzhen Key Laboratory for the Intelligent Microbial Manufacturing of Medicines, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.1School of Life Sciences, Inner Mongolia University, Hohhot, China.These dye kits are available in limited quantities or on demand since the dye production is painstakingly slow and as I said I am just processing what little amounts of snails I use for my own family consumption.Feifei Li 1,2,3,4, Huaxiang Deng 2,3,4, Biming Zhong 2,3,4, Banlai Ruan 2,3,4, Xixi Zhao 2,3,4* and Xiaozhou Luo 1,2,3,4* If preserved properly, murex dyes and pigments remain vibrant and shiny for centuries and even intensify with time as the dyed stuff keeps interacting with the elements (precisely sun light). Small samples of dyed silk and wool (10 different hues in total) A capsule of nearly pure purple pigment that was in ancient times worth several times its weight in gold (for instance up to 100 pounds of fresh snails are needed to produce less than 1g of more or less pure pigment)Ĥ. Some dried glands (the organ that actually yields the dye precursor inside the snail)ģ. A set of 3 murex shells the kinds historically used to produce the dye namely (from right to left in the attached photo) Purpura haemastoma, Hexaplex trunculus and Bolinus brandaris.Ģ. After years of experiments and research and at the demand of many of my friends around the world, I thought of putting together the main ingredients of this legendary dye in small elegant mini kits that would be ideal for workshops, lectures or presentations so that other people in other parts of the world can see and smell what these dyes looked like centuries ago!Įach of these mini wooden kits comes complete with:ġ.
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Sock Hops became a fad among American teenagers in 1948 and were commonly held at high school gymnasiums. In socks with stripes and socks with spots, socks with squares and socks with dots. We learned they were held as early as 1944 by the American Junior Red Cross to raise funds during the war. Duck Sock Hop comes once a week The mood is high, the sun is low, the music starts, get ready, go In this tongue-twisting, toe-tapping picture book, ducks grab socks from a big sock box and head on down to the duck sock hop. I thought Sock Hops were a thing of the 1950’s. It conatins accurate other and similar related words for sock hop in English. That number is expected to increase to 5,500 over the next several years. Abbey thought a Sock Hop would be a great theme for a party. Find sock hop synonyms list of more than 13 words on Pasttenses thesaurus. Joe's Livingston and Brighton perform over 4,600 surgical procedures each year, many of which are being performed on a recently acquired da Vinci® Si robotic surgery system. Joe’s Livingston and Brighton facilities. Joseph Livingston Hospital and Brighton Health Center and says the surgical service upgrades are part of a $41.1(m) million investment into St. Those services include construction of new operating rooms, the purchase of advanced technology, and the expansion of inpatient and outpatient surgical procedures being offered. Joseph Mercy Livingston Hospital in Howell and the St. Funds raised at the sold-out event will benefit the enhancement of surgical services at St. Using a festive 1950s’ theme, guests were encouraged to dress accordingly with plenty of leather jackets, poodle skirts and rolled up jeans making an appearance. Isolated cartoon vector legs of human comic feets in red shoes. Browse 50+ sock hop clipart stock illustrations and vector graphics available royalty-free, or start a new search to explore more great stock images and vector art. Isolated cartoon legs, human comic feet in shoes. Joseph Mercy Livingston’s Sock Hop Soiree was held Saturday at Crystal Gardens Banquet Center in Genoa Township. Browse 110+ sock hop cartoon stock illustrations and vector graphics available royalty-free, or start a new search to explore more great stock images and vector art. Money raised at a themed charity ball this past weekend will help bring new operating rooms, advanced technology and more surgical procedures to Livingston County. Unlike other dolls on the market who claim to be silicone, our dolls are authentic and a step above. Plus, our TrueTouch authentic silicone dolls are always offered at a great value when you consider how incredible and real they truly are. That's because their soft and supple skin lends a unique feel of "giving" when you hold them close to recreate the experience of holding a real baby. Our TrueTouch® authentic silicone baby dolls are some of the most sought-after bundles of joy offered at Ashton-Drake. TrueTouch Authentic Silicone Dolls Are So Lifelike We also offer a few toddler dolls that will walk with you when you hold their hands, letting you experience the pure joy of a little one's very first steps any time you like. They even "breathe" so you can see their tiny chest move gently up and down. And some of our interactive dolls have a "heartbeat" you can hear and feel when you put your hand on their chest. Some of them "coo" and "babble" to soothe their little selves as you hold them. Our interactive dolls actually respond to your touch. Interactive Dolls Respond to Your Touch and YOUĭid you know that some of our lifelike baby dolls have an additional layer of realism? It's true. Each So Truly Real doll has a unique personality that's reflected with their sweet hand-painted features, super soft skin, hand-rooted hair, a perfectly weighted body to make them feel just like a real baby and each doll arrives dressed in custom ensemble specially designed and made for them. Choose a precious little boy doll or girl doll (or even a cuddly monkey doll) to be the object of your affection and feel the warmth in your heart. In fact, once you hold one of these cuties in your arms, you might not want to put them down. The name of our most recognized brand of realistic baby dolls says so much about the wonder that awaits you: So Truly Real®. For all of the dolls in our nursery, we work closely with the world's most renowned Master Doll Artists to create adorable bundles of love meant to give you joy each and every day. And in addition to our baby dolls, we also have well-crafted portrait dolls. If you're giving it as a gift, pair your Precious Moments figurine with the right Hallmark greeting card and gift wrap to take it to the next level.Ashton-Drake Dolls: Experience the Differenceįor over 30 years, Ashton-Drake has been a leader in the world of dolls, combining unparalleled handcraftsmanship and award-winning artistry to bring you incredibly lifelike baby dolls, including toddler dolls, reborn dolls and newborn baby dolls. Shop for hundreds of figurines and other home decor items. Precious Moments Christmas ornaments adorning your tree provide an extra touch of holiday spirit for any collector, along with Nativity and other Christmas-themed figurines. Pair bride and groom figurines for a Precious Moments wedding cake topper. Whether you're offering encouragement, honoring the loss of a loved one with a sympathy gift, or commemorating a major life event like a graduation or anniversary, Precious Moments figures, picture frames, snow globes or wall plaques will put a smile on your face. The multitude of angels and other religious figurines as well as crosses make a gift perfect to commemorate a baptism or First Communion. Collecting the Disney Princesses or the animals in Precious Moments birthday train makes a fun yearly tradition. These hand-painted, sweet-faced figures bring to life favorite Disney characters, from Mickey Mouse and Minnie Mouse to Disney fairies. Their artistry and attention to detail make them a favorite for collectors around the world. With their enormous eyes and angelic expressions, the child-like porcelain figurines have been capturing hearts for more than four decades. Few collectible figurines are as widely known as Precious Moments. Otherwise, with young kids, it will happen. So unless fingers are on those 2 outside corner posts, there is no way that a kid can injure any fingers. You would have a post in the front 2 corners for the lid to rest on. You do this by leaving a 1” gap between the lid and the side panels and front panel. Left Spring Lid Support in Antique Brass. Anything smaller and it will strip out, i learned the hard way.Īlso, while designing the bench since it is for kids, design it so that when the lid is closed, there is no possibility of their fingers getting caught. Shop Gatehouse Antique Brass Lid Support Cabinet Hinge in the Cabinet Hinges department at . 806 101 Lung Chest Hinges.60 & 10060 & 10 & 105 Hinge Hasps. Note, for the cabinet member you must use a 5mm euro screw. This toy chest hardware is used in conjunction with hinges (or a piano hinge), these lid supports counterbalance the lid to keep it from slamming shut. Toy Box Hinges Shop Lid Stays, Lid Hinges and Lid Supports Finishing Products/Paint Sundries Call Toll Free: (800) 708-6649 Home Cabinet Hardware Lid Stays, Lid Hinges & Lid Supports Shop Lid Stays, Lid Hinges & Lid Supports - 154 items Eliminate slamming doors with lid stays, hinges and supports. I had 80 benches made for a nursing home and we used these hinges for all the lids, they worked great. Toy box lid support self-balancing feature holds lid in any position to about 85 degrees. What is linked below is the hafele maxi hinge. They sell the same hinge but different for various sizes and weights. Do not buy the hinge i have linked without figuring out how much your lid weighs and what its dimensions are. It is a lift assist hinge which makes the lid feel lighter than it is and also holds it in the upright position. Get great value for your money when you shop with us.įor more information about our toy box lid hinges, feel free to call us at 88 or email us today.Use a hinge like this that is linked below. In addition, we offer attractive discounts for bulk purchases of hinges and hardware. With years of experience behind us, trust Hinge Outlet to identify the best solutions for you. Our extensive product range contains everything you need for residential or commercial projects. Looks like real old oil rubbed bronze Great for finishing box lid projects, toy boxes, cedar chests, bench seats, doors, furniture, wood crafts and other wood. Hinge Outlet offers high quality hinges and accessories at attractive prices. Why Buy Toy Box Lid Hinges From Hinge Outlet? With so many options available, you’ll have no trouble finding the perfect hardware. For the lid to be flush with the back of the box, the top of the hinge should be 1-9/16 from the back edge. Toy Box Hinges Soft Close - Lid Support Hinges Heavy Duty,Soft Close Hinges for Chest Toy Box,Lid Stay Support 50lb/2pcs (APFFSY) 540. We also offer different lid supports that can be used on a variety of applications. Attach hinge to lid with 3/4 screws (included). You can easily compare and choose from the 10 best Slow Closing Toy Box. Our Balance Adjustable Spring Loaded Lid Stays feature a spring mechanism to hold lids in an open position from 10° to 80°. Best Reviews Guide analyzes and compares all Slow Closing Toy Box Hinges of 2023. Wooden Toy Box Kids Storage Chest Organizer W/ Flip-Top Lid & Safety Hinge White. In a toy comprising a receptacle, a plurality of blocks disposed in. They come with speed adjustment screws for easy adjustment. This toy chest hardware is used in conjunction with hinges (or a piano hinge), these lid supports counterbalance the lid to keep it from slamming shut. Shop Target for toy boxes and chests you will love at great low prices. A box according to claim 1, having the hinges for the end walls at the exterior of. Some of the products we carry include Adjustable Soft-Down Lid Stays that open to 70° or 105°. We offer a large selection of toy box lid hinges that are suitable for toy boxes and piano benches. That’s why they’re also called horizontal lift up hinges. Toy box lid hinges are used to secure horizontal lids. “Now, will they face challenges? Yes, they will. Robinson’s advice to females considering a career in engineering? “You are just as capable,” she declared. Bringing that skillset into the technical world is hugely important for us as a country to develop the kind of workforce we need to solve our nation’s problems.” Women are wonderful multi-taskers and problem solvers every day in life. “Women sometimes feel timid about going into a less traditional role and if I can provide encouragement for them to do that, it is hugely fulfilling and rewarding,” added Robinson. “We have a very small representation in what are considered to be male-dominated industries - engineering being one of them.” “One of the things I feel strongly about is encouraging young women to consider a technical role in their professional lives,” she said. However, Robinson’s career accomplishments serve as an inspiration for young females who desire to enter the profession. “Continuing to pursue space exploration is important to our global advancement as a society.”Īccording to Robinson, being a woman in field of engineering presents its fair share of challenges. It motivates the global workforce and gives us uniting causes where we as nations can work together for the good of humanity.” It stimulates advances in science and technology that help us all here on Earth. “We feel very strongly about that around here because it forges innovation and economic prosperity. “Our vision here at the Space & Rocket Center is to change lives and change the world through the power and passion of space exploration,” continued Robinson. Speak to Robinson and you will quickly realize the strong passion she holds for space exploration and how her organization mentors those seeking to make their mark on the industry. It’s a huge responsibility and inspiration.” “And to live up to that expectation and to actually touch people’s lives and develop the type of technical workforce that we need is incredible. “We have signs around this campus that reads, ‘Through these doors walks the next generation of future scientists, engineers, astronauts, etc.,'” she said. Space & Rocket Center plays in the development of future leaders in the field. Robinson is reminded daily of the vital role the U.S. To be given the keys to those kingdoms - it’s a big responsibility.” “We have an incredible array of artifacts and archival material that we are entrusted to keep. “I believe that we have to be good stewards of our funds and assets,” she said. It’s also a responsibility that I take very seriously.” “To be a small part of that is just incredible. I hear stories every day of how we’ve been able to impact people’s lives,” said Robinson. To be in a leadership position of any type at the miraculous U.S. Space & Rocket Center, which seeks to inspire individuals to pursue careers in space exploration, is rewarding for Robinson in that she has played witness to young professionals living out their dreams. Leading such a vital organization in the U.S. NASA leadership, recognizing the excellence in her work, entrusted her to lead utilization for all Artemis missions for agency HQs and Advanced Exploration Systems. This includes serving as a Propulsion Laboratory project engineer, astronaut trainer, project integration manager for the Ares 1-X test flight, and payload mission manager for Artemis I. In 1989, Robinson began her career at the Marshall Space Flight Center, where she served in numerous leadership roles. The nation’s chief space exploration agency has awarded Robinson with multiple honors, including an Exceptional Achievement Medal and the Silver Snoopy, which recognizes her achievements in human flight safety and contributions to mission success. in engineering management and systems engineering from the University of Alabama in Huntsville. A 31-year veteran of NASA, Robinson earned an M.S. Under most loan agreements, as a condition to borrowing a borrower must certify in writing that no MAE has occurred. Borrowers should nonetheless be aware of some important issues and considerations prior to drawing down a revolver.Ī gating issue for any borrower seeking to draw down on an existing line of credit is whether the borrower can meet the loan agreement’s borrowing requirements, in particular the “material adverse change” or “material adverse effect” (MAE) representation. Similarly, companies with sufficient cash for the near term are nonetheless considering drawing down on their revolvers as a precautionary measure to ensure they have enough cash on hand to meet obligations and outlast the COVID-19 crisis.ĭrawing down a revolver may indeed be one of the best ways for borrowers to meet current obligations and preserve their cash position as they ride out the adverse economic impact of the pandemic. As a result of this cash crunch, many companies with access to existing credit lines are considering whether to draw down on the unused availability to meet their current obligations. Meanwhile, the on-going obligations that companies incur in the ordinary course of business continue to accrue and remain due and payable. In this post, we examine one of these key issues in greater detail, namely drawing down on existing lines of credit, or ’revolvers’.Īs a result of the pandemic, many businesses are experiencing wide-spread revenue shortfall, and in some instances cash flow has ceased entirely. In a recent post, we examined the pandemic’s effect on corporate loan agreements and identified some key issues that borrowers should consider. Drawing Down on Existing Lines of Credit. Writing new private key to 'private_key.pem' Provide the details when prompted and ensure the Common Name matches the FQDN or the management IP address of the ~]# openssl req -x509 -newkey rsa:4096 -keyout private_key.pem -out selfsigned-cert.pem -days 3650 # openssl rsa -in private_key.pem -out private_key_nocrypt.pem # openssl req -x509 -newkey rsa:4096 -keyout private_key.pem -out selfsigned-cert.pem -days 3650 Generate a new self-signed certificate from a Unix host using the openssl command. Make sure the certificate file is transferred to the Splunk instance where the PureStorage Unified Add-on is certifi]# lsĬacert.pem core.py _init_.py _main_.py certifi]# cat /tmp/fa_pure.crt > cacert.pemġ. Open the $SPLUNK_HOME/etc/apps/TA-purestorage-unified/bin/ta_purestorage_unified/aob_p圓/certifi/cacert.pemfile in an editor and paste the certificate at the end of the file.Īlternatively, if you have downloaded the certificate, you can concatenate the certificate file to the cacert.pem file. Copy the certificate or download the certificate.ħ. Export the certificate that was just created from the SSL Certificate frame under the Settings=>System tab.Ħ. Accept it to establish a new secured connection.ĥ. After five seconds, the FlashArray GUI is reloaded prompting you with the warning message about the certificate. After providing all the details, click Create and select Yes to override the current certificate.Ĥ. The entry you are providing against Common Name should resolve to the Array either through DNS or the hosts file on the Splunk server.ģ. For someone new to the field of audio transcription, this may not seem like a big deal. Transcription foot pedals are a great way to control playback with your feet, leaving your hands free to only type. How to Use Express Scribe with a Foot Pedal At which point you’re better off giving your files to an audio transcription service like ours and have your peace of mind and time to spend on something more productive. If you’re not going to be doing this often, investing in a foot pedal makes no sense. So, a 1 hour audio file takes only around 6-7 hours instead of 8-10 hours without a foot pedal. A foot pedal can shave 1-3 hours off your total transcription time easily. Which is why professional transcribers or transcription services use a foot pedal to control playback. If your fingers leave the typing area and use the hot-keys to play, pause, rewind and forward etc. So, there will be a lot of going back and forth. Problem is you don’t catch everything at the first try, unless you’ve got superhuman typing and listening skills. You ideally want to spend most of your time typing during those 8-10 hours. To transcribe a 1 hour audio file with hot-keys, it takes about 8-10 hours, depending on your typing speed and proficiency. type in a document while listening, using hot-keys is definitely not the quickest or the most efficient way. Or if you want a free word processor, there’s the open source OpenOffice.īut if you want to transcribe, i.e. Using hot-keys works great if you’re proofreading a transcript using a word processing software like Microsoft Word. Hot-Keys are Great for Proofreading, but not for Transcribing However the software is more versatile than that and gives you tons of other options that we’ll further explore in this post. All Major Transcription Foot Pedal Controllers.Any Discrete Sound Card or Integrated Sound.To check how to use express scribe for the latest version, keep an eye on their website. Please note that this screenshot is of a recent version of Express Scribe. The screenshot below explains the various options you see in the Express Scribe interface upon installation.
Potato eyes are carved out without damaging your produce with an eyer that cuts through tough blemishes. No matter how wet it is, the soft grip does not slip. You can use this tool whether you're right-handed or left-handed since the handle is designed to be comfortable during fast, repetitive use. The knife flexes to peel large areas with each stroke and is made out of sharp, Japanese stainless steel. With the OXO Good Grips Vegetable Peeler, tough-skinned fruits and vegetables will be sliced and peeled in no time.
All dogs should initially receive one dose of this product and a second dose 2 to 3 weeks later. If blood enters the syringe freely, choose another injection site. IMPORTANT NOTE: Before injecting vaccine, pull back slightly on syringe plunger. Do not inject directly into blood vessel (see note below). Subcutaneous administration: Lift the loose skin behind the neck or behind the front leg and insert needle (see illustration, arrows 1 and 2). Withdraw entire contents into the syringe. Aseptically rehydrate the vaccine cake with 1 mL of sterile diluent supplied. Gentamicin added as preservative.ĭirections and dosage: Open the syringe by twisting or tapping the cap against a hard surface to break the heat weld. This product contains a CPV2b strain which has been demonstrated effective against disease caused by CPV2c in puppies with maternal antibody. For more information regarding efficacy and safety data go to .Ĭomposition: Canine Spectra ® 5 vaccine is a combination of immunogenic, attenuated strains of CDV, CAV2, CPIV, and CPV type 2b, propagated in cell line tissue cultures The CAV2 fraction has been shown to be effective against disease due to CAV1. The duration of immunity of this product has not been determined. Indications: This product has been shown to be effective for the vaccination of healthy dogs 6 weeks of age or older against canine distemper virus (CDV), infectious canine hepatitis (CAV1), canine adenovirus type 2 (CAV2), canine parainfluenza (CPIV), and canine parvovirus (CPV). Canine Distemper-Adenovirus Type 2-Parainfluenza-Parvovirus Vaccine |
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